Immunology Microplates

BRANDplates immunoGrade , hydroGrade , and lipoGrade microplates feature high-binding, hydrophilic and hydrophobic surfaces, respectively, for use in a variety of immunological applications such as ELISA, RIA, and FIA. Available in a full range of well shapes and colors in 96- and 384-well formats. 96-well immunoassay plates feature blue alphanumeric embossing for easy well identification.

immunoGrade
BRAND immunoGrade plates are optimized for the immobilization of IgG. They offer the highest binding capacity for molecules with mixed hydrophobic and hydrophilic regions. An excellent choice for the majority of standard ELISAs, and also suitable for solid phase immunoassays.

hydroGrade
BRAND hydroGrade plates feature a strongly hydrophilic surface, with a strong affinity to hydrophilic molecules, such as glycoproteins and peptides, antibodies with predominantly hydrophilic regions, and nucleic acids. They are an alternative to the immunoGrade plates for homogeneous assays with hydrophobic molecules that remain in solution.

lipoGrade
BRAND lipoGrade plates feature a strongly hydrophobic/lipophilic surface, for the immobilization of biomolecules with strongly hydrophobic regions. They are an alternative to the immunoGrade plates for solid phase assays, and are a choice for homogeneous assays with hydrophilic molecules that remain in solution.

Flexibility: BRAND immunoGrade microplates are available in a strip plate version for greater flexibility.

Frequently Asked Questions

1. How are the different BRANDplates surfaces produced?
The surface modifications of the microplates are produced via a physical-chemical process. This kind of surface modification creates durable surfaces that can be stored at room temperature.

2. How can I find the right plate for my application?
An overview of the BRANDplates available for different applications is available here. If you require further assistance in selecting the right plate for your application, contact BrandTech Customer Service at 888-522-2726.

3. What is the difference between a C-, F-, V-, and U-bottom plate?
BRANDplates 96-well plates are offered with four distinct well bottom plates, with different characteristics and advantages -

F-bottom: Flat well bottom. The F-bottom is designed for precise optical measurements and is well suited for colorimetric determinations as well as microscopy applications. Additionally, the F-bottom is used for specific applications such as cell culture.
C-bottom: Flat bottom with curved edges. The C-bottom combines the advantages of the U- and F-bottoms. Because of the curved edges, better sample mixing is obtained, while the flat bottom in the middle area of the wells allows precise optical measurements. The C-bottom is especially suited for immunological assays, as sample stirring has a major impact on precise assay results as well as the optical measurement to determine those results.
U-bottom: Round-shaped well bottom. Wells with a U-bottom have no edges, so they are well suited for stirring and washing of samples. This bottom shape is used for agglutination and other assays requiring these tasks.
V-bottom: Conically-shaped well bottom. Sample recovery is maximized with the V-bottom shape. For this reason plates with a V-bottom are especially suited for precipitation assays and storage.

4. What do immunoGrade , hydroGrade , and lipoGrade mean?
BRANDplates microplates for immunoassay are offered with three different surface treatments -

immunoGrade : The immunoGrade surface is optimized for the immoblization of IgG, offering the highest binding for molecules with mixed hydrophilic and hydrophobic regions. It is the surface of choice for the majority of standard ELISAs and is suitable for solid phase immunoassays.
hydroGrade : The hydroGrade surface is a strongly hydrophilic surface with high affinity to molecules such as glycoproteins and peptides, antibodies with predominantly hydrophilic regions and amino acids. It is a good choice for solid phase assays with hydrophilic molecules or liquid phase assays with hydrophobic molecules.
lipoGrade : The lipoGrade surface is a strongly hydrophobic surface for the immobilization of molecules with predominantly hydrophobic regions. It is a good choice for solid phase assays with hydrophobic molecules or liquid phase assays with hydrophilic molecules.

5. Are the BRANDplates microplates with immunological surfaces sterile?
No. Plates with immunological surfaces are not sterilized but are tested for the following criteria: Endotoxins

WARNING These products can expose you to chemicals including -Methyl styrene (alpha-Methylstyrene), which is known to the State of California to cause cancer, and Di-n-butyl Phthalate (DBP), which is known to the State of California to cause birth defects or other reproductive harm.
For more information go to www.P65Warnings.ca.gov.

BRANDplates for immunoassays, 96-well microplates -
Pack of 100 plates, 20 stacks of 5 plates (1 lid per stack)

Color	Bottom Shape	Well Volume	immunoGrade Cat. No.	hydroGrade Cat. No.	lipoGrade Cat. No.
Transparent	U-bottom	330 L	781720	781780	781840
Transparent	V-bottom	360 L	781721	-	-
Transparent	F-bottom	350 L	781722	781782	781842
White	U-bottom	330 L	781724	-	-
Black	U-bottom	330 L	781727	-	-

BRANDplates for immunoassays, 96-well transparent bottom microplates -
Pack of 100 plates, 20 stacks of 5 plates (1 lid per stack)

Color	Bottom Shape	Well Volume	immunoGrade Cat. No.	hydroGrade Cat. No.	lipoGrade Cat. No.
Black	F-bottom	330 L	781731	-	-

BRANDplates for immunoassays, 384-well standard microplates -
Pack of 50 plates, 10 stacks of 5 plates (1 lid per stack)

Color	Bottom Shape	Well Volume	immunoGrade Cat. No.	hydroGrade Cat. No.	lipoGrade Cat. No.
Transparent	F-bottom	100 L	781740	-	-
White	F-bottom	100 L	781741	-	-
Black	F-bottom	100 L	781742	-	-

Item Group: BRANDplates
Unit of Measure: Each
Pack Quantity: Pack of 100